Background: Jugular foramen is an irregular foramen at the base of the skull. Surgical approach to the jugular foramen has become very frequent in recent times due to the advancement in microsurgical techniques. Anatomical complexity of the jugular foramen makes surgical procedures thereon difficult. This requires thorough knowledge of morphological and morphometric anatomy of the foramen. Objective: To study the morphology and morphometry of the jugular foramen in dry human skulls of Southern Rajasthan region. Materials and Methods: The study was conducted in the department of Anatomy, RNT Medical College, Udaipur, Rajasthan. 100 jugular foramina (50 dry human skulls of unknown age and sex) were examined, and various measurements were taken with digital vernier calipers. Results: Mean transverse diameter of jugular foramen on right side was 15.25 mm and on left side 14.60 mm. Mean anteroposterior diameter of the foramen on the right side was 9.80 mm and on the left side 7.85 mm. The foramina were larger on the right side. Intra-jugular septa were incomplete in most of the foramina (95 percent) and complete in 5 percent. Jugular foramina were oval in78% and round in 22%. Conclusion: Comprehensive knowledge of anatomy of jugular foramen is of prime importance for radiologists and neurosurgeons as this area is laden with important neurovascular structures.