Background: The present study was undertaken for assessing antibiotic resistance profile of gram negative uropathogens isolated from a newly established tertiary care center. Materials &Methods: A total of 131 participants suspected for UTI were recruited using consecutive sampling techniques. Clean catch mid-stream urine samples were collected using sterile wide mouth container from enrolled patients. The minimum acceptable volume of urine sample was 10ml. As soon as the samples were brought to the lab, they were all examined to make sure that any pathogenic organisms found in the urine were isolated and prevented from becoming too large. Mueller-Hinton agar was subjected to antimicrobial susceptibility testing using the Kirby-Bauer disk diffusion method. Results:Enterobacter spp. were mainly susceptible to ampicillin, Chloramphenicol, Trimethoprim-Sulfamethoxazole and Amoxycillin. Citrobacter spp. were mainly susceptible to Ampicillin, Chloramphenicol and ciprofloxacin. Providenicia spp. were mainly susceptible to Ampicillin, Trimethoprim-Sulfamethoxazole and ciprofloxacin. Conclusion: Growing patterns of antibiotic resistance suggest that community antimicrobial usage must be rationalized and done so with caution.