Background: In minimally invasive surgery (MIS), intracorporeal suturing and knot tying (ICKT) is a crucial technique for complex procedures like the Roux-en-Y gastric bypass or Nissen fundoplication. The present study was conducted to assess intracorporeal suturing in single-port surgery. Materials &Methods: 58 surgeons of both genders who agreed to participate in the study were assessed for efficacy of intracorporeal suturing in single-port surgery Results: Out of 58 subjects, males were 30 and females were 28. Completion rate was 84%, 90%, 92%, 94% and 20%, execution time (minutes) was 60.2, 47.2, 43.2, 48.0 and 210.5. Stress was 4, 3, 4, 4 and 6 and precision was 5, 3, 5, 5 and 3 at manipulation angle of 90 degrees, 60 degrees, 45 degrees, 30 degrees and 0 degree respectively. The difference was significant (P< 0.05). Conclusion: Intracorporeal suturing in single-port surgery seems to be more difficult than conventional laparoscopic surgery.