Background: Surgical site infection in orthopedic implants is a major problem, causing long hospital stay, cost to the patient and is a burden on health care facilities. It increases rate of nonunion, osteomyelitis, implant failure, sepsis, multiorgan dysfunction and even death. Surgical site infection in orthopedic implants is more challenging to the treating orthopedic surgeon as the causative organism is protected by a biofilm over the implant’s surface. Antibiotics cannot cross this film to reach the bacteria’s, causing infection. Many bacteria have developed resistant to various drugs, and its frequency is Increasing day by day. Methodology Study design: Prospective cross sectional study. Study Centre: Bundelkhand Medical College, Sagar, Madhya Pradesh. Study duration: July 2021 to June 2022. Methods and material: All the post operative patients of Open Reduction and Internal Fixation at tertiary care centre, BMC, Sagar undergoing surgery and developing surgical site infection as diagnosed clinically by physician will be enrolled to study. SPECIMEN COLLECTION :- After taking written consent for sample collection, pus samples from all open reduction and Internal Fixation patient will be collected by sterile swab stick or syringes / suitable media. ISOLATION AND IDENTIFICATION of aerobic and anaerobic bacteria will be done by gram staining and culture in suitable media as per standard microbiological procedures along with suitable biochemical tests and antibiotic sensitivity test as per CLSI guidelines. Result: Total of 166 patients admitted to the orthopedic ward were approached, 19 patients were excluded, 9 patients refused to participate, and the remaining 138 patients were enrolled in the present study. The bacteria isolated and identified after laboratory culture. The single most common bacteria in the present study were Staphylococcus Aureus (54.3%) distantly followed by E. coli (18.1%) and Pseudomonas aeruginosa (12.5%). Among the 5 other bacteria, there were 3 cases of Staph. Epidermidis and 2 cases of Staph saprophyticus. Interpretation & conclusions: The most common location for the fracture was the distal femur (29.7%), followed by the proximal tibia (25.3%) and fracture of forearm bones (2.17%), fracture of humerus and metacarpal was the least common type of fracture. The single most common bacteria in the present study were Staphylococcus Aureus (54.3%) distantly followed by E. coli (18.1%) and Pseudomonas aeruginosa (12.5%). Among the 5 other bacteria, there were 3 cases of Staph. Epidermidis and 2 cases of Staph saprophyticus. 138 patients with fractures were included in our study; 64% were males and 35% were females, 65% of them had open or compound fractures, and the remaining 35% had closed fractures. So, for open/compound fractures, bacterial culture and sensitivity testing is done more.