Introduction: The SARS-CoV-2 pandemic has had significant impact worldwide, particularly in middle and low income countries. While this impact was well recognised in certain age groups, its effect on neonatal population is largely unknown. Although COVID-19 was described as mild disease in newborns, various concerns associated with the effect of infection on newborns are still there. Aim and Objectives: The aim of this study was to study the microbiological profile and COVID status of neonates having sepsis born to COVID-19 positive mothers in a tertiary care hospital. Material and Methods: Around 1000 clinically suspected cases of neonatal sepsis cases were reported among neonates born to COVID-19 positive mothers admitted to neonatal intensive care unit at a COVID dedicated tertiary care hospital during the period of July2021 to December2021. Around 2 ml of blood was collected and inoculated aseptically from the neonates into a brain heart infusion broth containing blood culture bottle. Identification of the organism was done conventionally on the basis of microscopy, colony characteristics and biochemical properties. Antimicrobial susceptibility testing was performed on all bacterial isolates as per CLSI guidelines. Results: Out of 100 neonates examined, bacteria was isolated from 80 neonates (80%), while rest showed yeast (Candida spp.) growth. The most common etiology for neonatal sepsis was Staphylococcus aureus species (20%), followed by Pseudomonas species (17%),Acinetobacter and CONS Coagulase-negative Staphylococcus (CONS (4%) each, followed by e.coli and Klebsiella (3%) each, Candida species were (20%),(Table 2) Of the 20 Candida isolates, non Candida albicans species were more common (66 %) than C. albicans (33%). Of these, Candida tropicalis (33%), Candida glabrata (16.5%), and Candida parapsilosis (16.5%) were the predominant speciesGram positive organisms showed most susceptibility to Doxycycline and Clindamycin while gram negative organisms to aminoglycosides and co-trimoxazole. All the 04 Candida isolates were non- albicans Candida. Conclusion: The current study suggested that there was no significance difference observed in the clinical presentation and outcome of newborns with sepsis and COVID-19 positive status versus non COVID-19 babies. But co-infection of COVID-19 virus and other microbes was observed here.