Background &Method: The aim of the study is to compare TNF-alpha and IL-6 as potential biomarkers for patients with diabetic neuropathy. Five ml blood was collected by phlebotomists by venepuncture from patients and healthy controls at the common collection centre. Blood was immediately transferred into 3ml green top heparin vial and 2ml blood into Purple top EDTA vial.Glycatedhemoglobin (HbA1c) levels and Biochemistry were determined for all patients and healthy controls at the clinical Laboratory auto-analyzer. CRP and Insulin assay were done on a fully automatic immunoassay analyser. Result: The chi-square statistic is 5.87. The p-value is .043131. The result is significant at p < .05.The chi-square statistic is 311.8023. The p-value is < 0.00001. The result is significant at p < .05.The chi-square statistic is 0.7364. The p-value is .390818. The result is not significant at p < .05, for Anthropometric analysis of T2DM cases and Control, Inflammatory mediators in the study group& Immunoassay analysis of study group respectively. Conclusion: Our data confirms that TNF-α, IL-6, inflammatory mediator i.e., cytokines, plays a positive role in the pathogenesis of T2DM and can act as early prediction biomarkers which can prevent T2DM in this population. Further studies on the wider range of these inflammatory mediators in association with other biochemical, immunoassay and hematological parameters are needed to establish role of inflammatory markers as early prediction biomarkers which can prevent T2DM in this population. Keywords: TNF-alpha, IL-6, biomarkers, diabetic & neuropathy.