Introduction: This study was planned with objective to evaluate magnitude of anxiety and depression using HADS score and associated factors among healthcare workers during COVID-19 pandemic at a tertiary care hospital. Methodology: This cross sectional study was conducted among health care workers at a dedicated COVID hospital in Jaipur from July 2020 to June 2021 during COVID- 19 pandemic. All consent giving participants of age ≥18 years were enrolled in this study excluding HCW having pre-diagnosed mental health illness or those who did not gave consent. Anxiety and depression scores were calculated using HADS scale and were analyzed using SPSS version 20.0. Results: In this study, 100 health care workers were enrolled. The largest proportion (78%) were doctors followed by nursing staff (11%) and Class 4 employees (11%). Maximum 70 (70 %) participants were remain admitted both in ward and ICU. Proportion of anxiety was 88% and depression was 23% among HCW. The mean anxiety score was 10.36 ± 2.052 and the mean depression score was 6.62 ± 1.841 in the HCW group. Male HCWs had higher mean anxiety score (10.44 ±1.980) (p=0.567) and higher Depression score (6.72 ±1.852) (p=0.429).Mean anxiety score was higher in 21-40 years age group (10.52± 2.002)(p=0.689).In contrast, mean depression score was higher in >60 years age group (8.33± 1.875) (p=0.002).Doctors were having highest Anxiety score (10.55± 1.898)(p=0.043) and highest depression score (6.92± 1.864)(p=0.002).Those admitted in ward had maximum anxiety score (11.8±1.322) (p<0.001) and maximum depression score (6.75±1.86) (p=0.182). Conclusion: Mental health of Health care workers was severely affected during COVID pandemic. Higher anxiety and depression scores were observed specially among doctors. Efforts should be made to promote mental health.