Background: Helicobacter pylori (H. pylori) is a type of bacteria that can infect the stomach and the upper part of the small intestine. The present study was conducted to assess association between increased neutrophil lymphocyte ratio and erythrocyte sedimentation rate in patients with Helicobacter pylori positive chronic gastritis. Materials & Methods: 58 patients with H. pylori infection of both genders were kept in group II and healthy subjects in group I. Patients with positive HP were subdivided in two groups of 29 each according to gastritis staging. 5 ml venous blood samples obtained in the morning after 8-hours fasting and the measurement of NLR and ESR levels were performed. Results: In group I, males were 29 and females were 29 and in group II, males were 30 and females were 28. In group I and group II, mean hemoglobin level was 13.1 g/dl and 12.8 g/dl, platelet count (×1,000) was 292.5 and 278.4, WBC was 8524.2 and 9462.3, neutrophil count was 6542.8 and 8024.6, lymphocyte count was 2304.2 and 1425.6 and NLR was 2.84 and 5.34 respectively. ESR was 20.4 mm/hour in group I and 36.2 mm/hour in group II. The difference was significant (P< 0.05). The mean NLR in asymptomatic gastritis group was 5.28 and in symptomatic gastritis group was 6.94. The difference was significant (P< 0.05). Conclusion: There is an increase in NLR as the severity of gastritis with HP increases. NLR is the strongest markers of inflammatory condition.