Background: Entamoeba histolytica/ Entamoeba disparare two morphologically indistinguishable species and can mimic symptoms of inflammatory bowel disease. This infection can be acquired through the consumption of ntaminated food or water and has a high chance of flaring up due to already damaged intestinal mucosa. Our study aims to find the incidence of this parasite in patients of IBD so as to differentiate between these two closely symptomatically resembling diseases ultimately benefitting the general public by timely diagnosis and also a gender differentiation if present. Methods:100 patients of IBD were enrolled in the study and routine microscopy of stool, aspirates of pus in case of liver abscess were performed. Approximately 4 ml of blood was taken under aseptic precautions and serum was separated and subjected to E. histolytica/ E. disparIgG ELISA. Results:37 patients (37%) were positive for cysts in their stool and out of these, 18 patients (48.64%) also came out to be positive for E. histolytica/ E. disparserum IgG and remaining 19 patients (51.35%) were negative. Among these 18 patients, 13 were males (72.22%) out of which only 2 males had liver abscess with cysts in routine microscopy of pus aspirates and 5 were females (27.78%) without liver abscess. Conclusion:It was observed that the incidence of E. histolytica/ E. disparwas significantly higher among males than in females with IBD which can be due to the fact that the male population usually belongs to the working class and daily wage workers in a district like Gwalior wherethe rural population is dominant as compared to the urban population who are devoid of proper infrastructure for waste disposal and sanitation measures and a general lack of awareness about the spread of the disease.