Background: Lipoprotein is a biochemical assembly that contains protein and lipid. Lipids are the derivatives which covalently or non-covalently bind to the proteins. Objective: To Study the cord blood lipid profile in neonates [Total cholesterol (TC), Low density lipoprotein (LDL), High density lipoprotein (HDL) and triglyceride (TG)] at birth using umbilical cord blood. Methods: The present prospective study was carried out on neonates born at Dr. TMA PAI HOSPITAL, UDUPI. This study Included screening of 137 newborn babies. Results: There were 76 males & 61 female with (55% male and 45% female) with gender ratio of 1.24: 1. In this study we studied total of 137 newborn’s out of which majority i.e 128 were appropriate for gestational age (AGA), small for gestational age ( SGA) were 4, Large for gestational age (LGA) were 5. TG levels were higher in SGA babies in the order SGA > AGA > LGA babies. TC levels were higher in LGA babies in the order LGA > AGA > SGA babies. LDL levels were higher in LGA babies in the order LGA > AGA > SGA babies (Table 4) HDL levels were higher in LGA babies in the order LGA > AGA > SGA babies. In present study mean TG level of SGA group (68.25 ± 33.5) was significantly higher as compared to AGA group (42.36 ± 14.3) (p value = 0.001) however falls between 50th and 95th percentiles of cord blood lipid values for the age. Conclusion: LGA babies have higher levels of total cholesterol, LDL, HDL than appropriate for age babies and small for gestational age however it falls within 50th percentile for cord blood reference LDL levels for that age.