Introduction: Enterococcus species are normal residents of the gastrointestinal, biliary tract, vagina and male urethra. Enterococcus faecalis has recently become the major pathogen exhibiting resistance to many antimicrobials which are commonly used with increased frequency to treat infections. The aim of this study was to determine prevalence and antimicrobial susceptibility pattern and Vancomycin resistant Enterococcus faecalis in patients attending a tertiary care hospital in southern Rajasthan. Material and methods: This study was conductedon 800 clinical samples in the Department of Microbiology, Pacific Medical College and Hospital Udaipur, Rajasthan, from January 2020 to January 2022.The samples included urine, blood, sputum, ascitic fluid, Pleural fluid, Pus, ET secretion collected aseptically from patients suffering from urinary tract infection (UTI), septicaemia, pyogenic infections and their culture and antibiotic sensitivity were performed as per standard recommendations of CLSI guidelines (Clinical and laboratory standard Institute) by disc diffusion method. Results: In our study, Out of total 800 samples, 109 samples were positive for the Enterococcus faecalis that form13.62 % of all samples. Similarly 17 samples were positive for Enterococcus faecium which form2.12% and 15 Enterococcus species were unidentified. Enterococcus faecalis isolates were 22.93%, 18%, 10%, 8%, 22%, 22%, 6%, 20 % of urinary, wound swab or pus, blood, ascitic fluid, sputum, pleural fluid and ET secretion samples respectively. Out of 109 of Enterococcus faecalis 29(26.60%) samples were Vancomycin resistant. Conclusion: Various studies have shown an increase in the rate of infection and antibiotic resistance in Enterococcus species. There is also a change in pattern of antibiotic resistance in Enterococcus species with an increased isolation rate of VRE. The outdoor patients have community acquired Enterococcus infection.