Introduction: Neonatal sepsis is a blood infection that occurs in an infant younger than 90 days old. Early-onset sepsis is seen in the first week of life. Late onset sepsis occurs after 1 week through 3 months of age..It is one of leading cause of morbidity and mortality. Therefore, early diagnosis and provide good management prevent fatal outcome. C-reactive protein is an important biomarker that aids in the timely diagnosis of neonatal septicaemia. Aim: The present study was carried out to evaluate the CRP in the diagnosis of neonatal septicaemia with blood culture. Materials and Methods: 221 neonates diagnosed with neonatal sepsis were included in the study over a period of 1year . Blood culture and semi- qualitative assessment of CRP was done for all the patients. Results: Of the 221 neonates studied, 90 were blood culture positive while 131 were CRP positive. The sensitivity, specificity, positive and negative predictive values and diagnostic accuracy of CRP were 86.7%, 43%, 45.5%, 85% and 69% respectively. Conclusion: The specificity and sensitivity of CRP against blood culture strengthen the use of this acute phase protein in the diagnosis of neonatal sepsis.