Background: Saliva has several diagnostic applications and is useful in both elderly and young patients. The present study was conducted to compare salivary glucose and blood glucose levels in diabetic and healthy subjects. Materials & Methods: 46 diabetes mellitus and healthy subjects were selected. 5 ml of venous blood was collected for estimation of plasma glucose, fasting blood sugar (FBS) and postprandial blood sugar (PPBS) level. Estimation of glucose levels from the serum and supernatant saliva was done by using the glucose oxidase-peroxidase method (GOD-POD method). Results: In group I males were 26 and females were 20 and in group II, male and females were 23 each. The mean FBS level in group I was 165.2 mg/dl and in group II was 76.4 mg/dl. The mean PPBS level I group I was 216.4 mg/dl and in group II was 86.2 mg/dl. The mean salivary glucose level in group I was 13.2 mg/dl and in group II was 5.8 mg/dl. The difference was significant (P< 0.05). Conclusion: There was increased salivary glucose levels in patients having diabetes mellitus. Salivary glucose levels could serve as a potentially non-invasive adjunct to monitor glycemic control in diabetic patients.