The investigation was conducted with methanolic leaf extract of Murraya koenigii for its antibacterial and cytotoxic activities. Antibacterial activity of the extract was evaluated against various Gram-positive and Gram-negative bacteria using disk diffusion technique. For cytotoxic activity, brine shrimp lethality bioassay was performed to estimate LC50 values. In our preliminary screening, the n-hexane, ethyl acetate and dichloromethane soluble fractions of the crude methanolic extract of Murraya koenigii were subjected to antibacterial activity and brine shrimp lethality bioassay. The ethyl acetate and dichloromethane soluble partitionate of the methanol extract exhibited the mild to moderate antibacterial activity. The ethyl acetate extract showed antibacterial activity against Bacillus subtilis and Staphylococcus aureus (Gram positive), and dichloromethane extract showed antibacterial activity against Pasteurella multocida and Proteus vulgaris (Gram negative). And the n-hexane soluble partitionate exhibited strong cytotoxicity having LC50 of 1.250 g/mL.