Present study aimed to evaluate the microbiological flora in diabetic foot based on a study of 200 patients. A prospective study of 200 patients with diabetic foot who were admitted to Surgery ward of Zydus Medical College & Hospital over a period of one year. All patients underwent microbiological analysis of their wounds, including bacterial and fungal cultures. Results showed that 180 out of 200 patients (90%) had positive microbiological cultures. The most common bacterial species isolated were Staphylococcus aureus (42%), Streptococcus spp. (19%), and Enterococcus spp. (12%). Gram-negative bacteria were also present, including Escherichia coli (9%), Pseudomonas aeruginosa (7%), and Klebsiella pneumoniae (4%). Methicillin-resistant Staphylococcus aureus (MRSA) was identified in 23% of patients. We concluded that the significant role of microbiological flora in diabetic foot infections. Bacterial and fungal colonization was present in a high proportion of patients, with Staphylococcus aureus and Candida spp. being the most prevalent microorganisms. Our findings also suggest that antibiotic resistance is a growing concern in the management of diabetic foot infections. Effective treatment and prevention of diabetic foot require a comprehensive understanding of the microbiological factors involved, including the use of appropriate antimicrobial agents and wound management strategies. Keywords: Diabetic Foot, microbial flora.