Aim: To determine the effect of antiepileptic drugs on serumvitamin- D3 levels in newly diagnosed epilepsy patients. Material and methods: Total 65 patients (OPD/IPD) aged between 12-45 years who were newly diagnosed and matching against the inclusion and exclusion criteria were included in the study. Those who were found deficient at baseline were excluded from the study .Patients were assessed for vitamin D deficiency at further follow ups of 3rd and 6th month. Results: We observed that vitamin D levels were found low at 3rd month as compared to baseline while after the start of vitamin D3 supplementation there was increase in the levels at 6th month as compared to 3rd month. Patients on polytherapy had greater decrease in vitamin D than on monotherapy. The total decrease in vitamin D levels in patients taking EIAEDs and combination was significantly more than in NEIAEDs at 3rd month. Conclusion: In our study it was observed that, antiepileptic drugs leads to significant reduction in vitamin D level. So there is need to monitor serum vitamin D levels periodically during anticonvulsant therapy so that therapeutic Vitamin D should be started as early as possible which would eventually leads to better quality of life of suffering patients. Keywords: Vitamin D, epilepsy, monotherapy, polytherapy, supplementation