Background: Vertical root fractures in endodontically treated teeth have been found in good percentage of cases and must be diagnosed early to prevent the damage of supporting structures of teeth. Clinical and radiographic findings being non specific warrant the use of advanced diagnostic aids like cone beam computed tomography for early detection and management of vertical root fractures. Aim: To detect the diagnostic viability of Cone Beam Computed Tomography in presence of intra-radicular obturating materials in human teeth. Methodology: Human mandibles with intact teeth were used in which teeth ere atraumatically extracted, root canal preparation done and incomplete fractures induced in half of the sample teeth. CBCT scans were taken before obturation and after obturation of root canals to compare sensitivity and specificity. Results: Results showed that presence of radio dense intra canal obturating materials results in decrease of both sensitivity and specificity of detecting vertical root fractures with high rate of false positives due to streaking artefacts from obturating materials.Conclusion: Presence of Intracanal radiopaque materials decreases the reliability of CBCT as a diagnostic tool in detection of vertical root fractures of teeth.