Background:The outbreak of COVID-19 has underscored the need to explore gender-specific preparedness and response strategies, as the reverberations of the pandemic are contingent upon both social and physical factors. Hence, this study aimed to elucidate the demographic and clinical characteristics of COVID-19 infection among female healthcare workers (HCW) in a tertiary care hospital in Tamil Nadu, India.Methods:A total of 980 healthcare workers were included in the study, and data were collected from 1st May 2020 to 31st April 2021. The prevalence of COVID-19 infection was determined, along with an examination of the distribution of infected cases among different professional categories. Symptomatology data were analyzed along with the prevalence of comorbidities among the infected individuals. Data analysis was conducted using SPSS version 21.0 to identify trends and patterns related to COVID-19 infection.Result:The analysis revealed that 16.5% (95% CI: 14.3-19.1) of female healthcare workers within the institution were infected with COVID-19 since May 2020. Among the infected cases, nurses accounted for 47.8%, doctors for 33.8%, and other healthcare workers for 18.4%. The most prevalent symptoms among the infected individuals were fever (28%), cough (25%), and myalgia (22%). The incidence of infection declined after vaccination with 14.3% before vaccination and at 2.3% after vaccination.Conclusion:Comprehensive protection measures against the pandemic should be extended to all healthcare workers, ensuring that no one is left behind. By addressing the unique challenges faced by female healthcare workers, we can strengthen their resilience and contribute to the overall containment of the outbreak.