Introduction- Stressors for premature infants in the NICU include external stimulation with sounds, light, touch, and open positioning that have a negative impact on outcomes. The aim of the present study was to measure the effectiveness of a developmental positioning intervention on length of stay, weight gain, and tone/flexion compared with neonates without structured positioning. Material & methods- Preintervention sample was provided by 60 newborns with inclusion criteria of 34 weeks of gestation or fewer and no anomalies in the study's quasi-experimental design. The researcher employed the Infant Position Assessment Tool as a visual reference for positioning and rating intervention fidelity. Results– In comparison to the control group, the postintervention group was both sicker and younger (P <0.05). The preintervention sample (M = 7.21) was older than the postintervention sample (M = 7.07). The preintervention sample (M = 1376g) was larger than the postintervention sample (M = 1301g). The post-intervention group's weight gain was clinically significant, and the higher mean Hammersmith score (3.47) indicated that positioning had a beneficial impact on tone and flexion scores. Conclusion- Results are improved with more organization and constant focus on developmental positioning. Stronger connections and relationships between posture and outcome measures will be revealed by additional study with larger sample numbers.