Background: Arthroscopic reconstruction of the injured ACL has become thegold standard.The aim of this study to compare the functional outcome of arthroscopic reconstruction of anterior cruciate ligament using patellar tendon bone grafts v/s hamstrings graft at multicentre tertiary care center. Materials& Methods: This prospective randomized controlled trial study was conducted at Orthopaedic Department in multi center tertiary care hospital in Rajasthan during one year period. A total of 60 patients with age above 20 years had unilateral anterior cruciate ligament rupture were enrolled.All sixty patients were divided into two groups; Group I consist of 30 patients those received patellar tendon graft by open technique and Group II consisted of 30 patients who received semitendinosus and gracilis quadruple tendon graft arthroscopically. Anterior kneeling pain during pray or labor was examined between both groups at six months, 1 year, and at the final follow-up. Patient’s satisfaction and VAS score were recorded at the final follow-up. All the data were analyzed by SPSS 24. Chi-square test and student t’ test was applied to compare the outcomes with a p-value of<0.05 considered statistically significant. Results: In Group I, patients presented with a mean age of 39.24±10.74years while in group 2 mean age was 38.78±11.56years. In group I and Group II, preoperative Lysholm Score were 62.58±4.45 and 63.46±4.56, IKDC was 3.65±0.28 and 3.82±0.55. At final follow-up we found no significant difference in term of Lysholm score, IKDC score and painscore was 88.24±2.07 Vs 89.68±1.42 (p>0.05), 1.86±0.66 Vs1.28±0.35 (p>0.05) and 7.87±0.62 vs 8.03±0.24 [p>0.05]. When kneeling pain was assessed, 10, 6, and 2patients in group I had kneeling pain during working or praying at 6months, 1year, and at the final follow-up respectively, while in group II, 11, 6, and 1patient had kneeling pain at 6 months, 1 year, and at the final follow-up respectively. Conclusion: We concluded that both techniques patellar tendon and hamstrings autografts are safe and effective treatment modalities for anterior cruciate ligament reconstruction.