Neonatal screening programs for detection of CH in neonatal period are widespread in the developed countries for the last three decades and are fast gaining momentum in the developing world as well. In most screening programs for CH, blood samples are collected at 5 - 6 days of life. Because of large number of babies being discharged early, cord blood samples are being used as an alternative. Blood samples were collected in a sterile container drawn from a 15-20 cm length of the umbilical cord incised while severing it at the time of birth of the baby. Thus, a mixed cord blood sample (1.5 ml) including both from the umbilical artery and vein was obtained. The level of thyroid hormones in the cord blood samples of the 1550 neonates of this study were T3: 72.69±13.12 (ng/dl): T4: 9.01±2.227 (µg/dl) and TSH: 8.64±6.195 (mIU/l).