Probiotics positive effects on disorders like constipation, protection from infection, necrotizing enterocolitis, diarrhea, as well as capacity to modulate immuneresponces have been demonstrated earlier without any side effects. Lactobacilli are classified as“generally regarded as safe” category by WHO. Also, FDA (Food and drug administration) has approved use of Lactobacilli and Bifidobacteria in infants. We divided the study infants into group A (Probiotics and Prebiotics combination) and group B (Simethicone, Dill oil and Fennel oil combination). Number in each block (block size) was calculated using the formula-2n (where and is number of groups). So, we got block size-4. As the total sample size was 96(we recruited 76), number of total blocks-19 for the recruited sample. All possible allocation sequence were assigned to numbers 1 to 6 For ex, ABAB-1, AABB-2, ABBA-3. In our study there is significant increase in night time sleep at the end of study (mean 6.539 and 5.816 with p=0.011) in Probiotics, Prebiotics combination group. Daytime sleep was similar in both groups; however, Simethicone, Dill oil, Fennel oil combination group shows less daytime sleep on day7 which is statistically significant. The increase in nighttime sleep may be due to decrease in mean crying time and less crying episodes from day3 onwards in Probiotic, Prebiotic combination group.