Background: Chronic periodontitis in a multifactorial inflammatory disease which is caused by various microorganisms. Many studies have found close association between chronic periodontitis and C-reactive protein (CRP). CRP is an inflammatory marker which increases in all inflammatory condition. Aims and Objective: The present clinical study was designed to show the effect of periodontal treatment on the CRP levels of gingival crevicular fluid and to determine the effect of nonsurgical therapy in minimizing the CRP levels in chronic generalized periodontitis. Material and Method: Gingival crevicular fluid was collected using a micro capillary pipette that was hand calibrated at every 1 mm till 10 mm, from selected sites in the subjects on the 1st, 14th and 45th days. Results: Bleeding index showed an improvement of 61% and 72% on the 14th and 45th days, respectively. Probing depth decreased by 29% on the 14th day and 43% on the 45th day. Similarly, the clinical attachment level showed improvement of 35% and 48% on the 14th and 45th days, respectively. Conclusion: The findings support the underlying inflammatory component of the disease activity in chronic periodontitis by demonstrating that the presence of CRP is more substantial in gingival crevicular fluid.