This work highlights the effects of Sodium Lauryl Sulphate on growth and physiology of the aquatic plant (Hydrilla verticillata). The effects were observed at five different concentrations. The experimental set up was laid within the open space of the Department of Environmental Studies, GIS, GITAM University. The duration of the experiment was 24 days. The results showed significant effect of sodium lauryl sulphate on plant's morphology (browning of shoots, fragmentation and leaf shedding), chlorophyll and protein content. The effects on chlorophyll and protein were apparently significant at higher concentrations (4-20 ppm). There was no significant difference between plants in control set and plants exposed to 2 ppm concentration of surfactant with respect to various parameters considered in the present study.