Background: This study was conducted for comparing the impact of desensitizing agents on the retention of crowns cemented with luting agents. Materials & methods: We selected 100 recently extracted mandibular molar teeth. All of the teeth were kept in regular saline pending usage. To remove surface stains, each specimen underwent a thorough cleaning process. The samples were then kept at room temperature in distilled water. A metal mold containing auto-polymerizing acrylic resin was partially filled with all of the specimens. The specimens were kept in distilled water for storage. By creating a clamp that could hold a high-speed air-rotor hand piece, uniform taper was achieved. Two research cohorts were formed: Glass ionomer cement is the control group in Group A, while GC Tooth Mousse desensitizer is the study group in Group B. Results: 100 newly extracted molars were included in the trial, and they were roughly split into two study groups: Group A received glass ionomer cement (the "Control"), and Group B received glass ionomer cement (the "GC Tooth Mousse desensitizer"). The mean tensile bond strength of group A specimens was 50.2 kg, whereas group B specimens' mean tensile strength was 49.4 kg. Results from the statistical comparison produced non-significant results. Conclusion: Desensitising chemicals may be used while crowns are being made because they won't influence the luting cements' capacity for retention.