Background and Objectives: Infraorbital foramen (IOF) is an important anatomical structure that is present on maxillary bone of both sides through which the infra orbital canal provides passage to infra orbital artery, vein and nerve and communicates with the facial structures superficially. Infraorbital nerve block is widely used to achieve regional anaesthesia during surgeries involving the midface region and paranasal sinuses. However, the anatomy of IOF varies between genders and among different population groups. The present study was conducted to study the dimensions, orientation, and position of IOF in relation to different clinically important anatomical landmarks of Indian skull.Methodology: The presentstudy was conducted at Anatomy department of our medical college overtwo years fromAugust 2020 to July 2022on skulls of known sex and agewith no apparent gross pathology, deformity, or traumatic lesions. Shape, size, number, direction of opening of IOF as well as distance of IOF from important anatomical landmarks of face were recorded on both sides of the skull.Results: 28 dry human skulls or 56 sides were studied. The commonest location was found to be in line with upper second premolar tooth (60.7%) followed by between upper first and second premolar tooth in 23.2%.The most common shape of IOF was oval (42.9%) followed by semilunar (28.6%). The commonest direction of opening of IOF was medially downward (51.8%) followed by medially (33.9%). In majority (73.2%) cases IOF was located lateral to the lateral margin of SOF. Mean vertical and transverse diameter of IOF were 3.16± 0.77 mm and 3.27± 0.78 mm on right side and 3.19±0.76 mm and 3.27±0.77 mm on left side respectively. Mean distance between IOF and IOM was 7.27± 1.48 mm, IOF and PA was 17.07±1.75 mm, IOF and LAM was 25.51±1.93 mm, IOF and SOF was 43.76±4.41 mm, IOF and ANS was 34.96±3.62 mm, IOF and Na was 42.57±4.15 mm. There was no statistically significant difference between males and females among the parameters studied.Conclusion: The morphology of IOF is quite variable, so it is essential to perform a meticulous preoperative evaluation of IOF in all patients posted for maxillofacial surgery and regional block anaesthesia. The anatomical landmarks studied could help in near precise localisation of IOF thereby decreasing the risk of failures and complications during procedures.