Background: An increase in the prevalence of the proximal humeral fractures which provides an accurate assessment of the various measurements of the bicipital groove and for assessing the gender differences on the same side of bicipital groove along with the difference between various sites on the same gender is vital. Aim: The present study aimed to reassess the morphometry of the bicipital groove using the radiographic method. Methods: The present study assessed 620 MRI (magnetic resonance imaging) scans that were done at the Institute within the defined study period. These MRI scans were from 310 males and 310 female subjects. Also, 380 scans were from the right side and 240 MRI scans were from the left side. The data gathered were assessed statistically for formulation of the results. Results: The study results showed a statistically significant difference in the genders concerning the width and depth of the bicipital groove and distance between lesser and greater tubercles. Additively, a significant difference was seen I different sides concerning the distance between tubercles and depth of the intertubercular groove. Conclusions: The present study concludes that resulting from the variations in the different population, attaining an accurate morphometry of the proximal humerus is now vital to simulate the anatomy of the proximal humerus. Also, the difference between the intertubercular grooves in the two genders can be helpful in forensic cases.