Introduction: Transfusion transmissible infections (HIV, HBV, HCV, syphillis and malaria) are among the major threats to the safe blood supply for the patients requiring blood transfusions. Seropositivity rate of TTIs among blood donors is a useful source of information to check their seroprevalance in a community which can give us accurate estimates of risk of TTIs, essential for monitoring safe transfusion services. Aim: To estimate the seropositivity of Human Immunodeficieny virus (HIV 1 & 2), Hepatitis B surface Antigen (HBsAg), Hepatitis C virus (HCV), Syphilis and Malaria among blood donors of a tertiary care teaching institution in north India. Materials and methods: This retrospective study was done from January 1 2022 to March 31, 2023 in Department of transfusion Medicine at a tertiary care teaching institution of north India. The results of serologic markers for Transfusion transmissible infections (HBsAg, anti-HCV, anti-HIV, syphilis and malaria) of all blood donations (both voluntary and replacement) at our hospital were collected from departmental records. Collected data was tabulated in Microscoft Excel and results were expressed as percentage. Results: Out of total number of 6505 donos, male donors were 6452, while 53 were female donors. 4213 donors out of 6505(64.76%), were repeat donors. There were total 160 seropositive donors, all of whom were male (100%) and 136 (85%) were replacement donors. There were no female reactive donors (0%). The highest number of TTIs were seen in 26 to 35 age group (41.87%), followed 36 to 45 years old (26.87%). The overall seropositivity rate of TTIs was 2.46% (160/6505). There were 50 donors who tested positive for HBV, 48 for HCV, 11 for HIV, 51 for Syphilis, and none for Malarial parasite. Conclusion: The current study found a low level of TTI seroprevalence in donor pool, indicative of a low overall level of TTIs in the population. Stringent donor screening and recruiting more of voluntary blood donors is need of hour for providing safe transfusion services.