Introduction: Bloodstream infection (BSI) is a serious problem in hospitals all over the world. It is defined by a positive blood culture in a patient with systemic signs of infection and may be either secondary to a documented source or primary—that is, without identified origin. It is the most common preventable cause of mortality and morbidity in critically ill patients. Aim: to identify the bacterial isolates from BSI in ICUs. Methods: The present prospective study was conducted in the Department of Microbiology for one year, in which patients admitted in ICUs with blood culture positive were included. Under all aseptic conditions ,sample was collected and processed as per standard protocolos Once the blood culture bottle flagged positive smears were prepared and sub cultured on blood agar and MacConkey's agar plates and incubated at 37°C for 18-24 hours. Identification and sensitivity of the organisms was done by using VITEK 2 system. Results: Total 226 patients were positive with BSI. The average age of the patients enrolled was 49.7±15.7 years. Majority of patients were males 73.9% and 26.1% were females. Monomicrobial growth was seen in 222(98.4%) and polymicrobial growth (two isolates each) was seen in 4(1.6%) samples which makes total of 230 bacterial isolates out of which 172 (74.8%) were Gram negative and 58(25.2%) were Gram positive isolates. Gram negative isolates were highly susceptible to amikacin and less susceptible to fluoroquinolones. Most of the Gram positive isolates were susceptible to linezolid, daptomycin, vancomycin. Conclusion: BSI with Gram negative organisms were more than that with Gram positive organisms.