Background: According to the WHO reports, each year approximately three million of the overall 35 million Healthcare Workers worldwide experience percutaneous exposure to blood borne viruses resulting 66 000 Hepatitis B infections, mainly from low-income countries.3 Healthcare workers/Providers(HCW/HCP) are known to have 2-4 fold risk of hepatitis infection compared to the general population.4.An additional risk factor for the acquisition of HBV among HCWs is the underlying prevalence of HBV infection in the population. Aims and objectives: To study the Knowledge, Approach & Practice assessment of Primary Health Providers towards Hepatitis B virus infection in Medical college.Material and methods: A cross-sectional study was conducted which includes questionnaire survey administered to all Health care providers working in different departments of Pacific institute of Medical sciences, Udaipur, Rajasthan (2020) and United Institute of Medical Sciences, Prayagraj, U.P. (2022) who come in contact with patient /client s body fluid / organ etc and are at a greater risk of acquiring blood borne infections. The questions are obtained from a various studies performed and modified. A questionnaire will be self-administered consisting of 4 parts including demographic data of participants, knowledge, approach and practices (KAP) of primaryHCW regarding the various aspects of HBV. Data compilation and statistical analysis will be done.Results: 97% participants agree with that hepatitis B is transmitted by contaminated blood and body fluids, Hepatitis B virus is spread by casual contact such as hand shaking agree 94%, Hepatitis B virus can be transmitted by unsafe sex (95%), Hepatitis B virus can be transmitted by unsterilized syringes, needles or surgical instruments (95%), Semen,saliva & vaginal secretions are modes of HBV transmission (96%), 79% participants agree with that chronic infection with viral hepatitis B is shameful, 84% feel confident in dealing with a patient who is HBs Ag-positive, 81% recommend the mandatory HBV vaccination for all the health care Providers including both the Medical colleges, 80% accept a colleague with Hepatitis B virus in the same work place, 84% were agree with that training programs for Hepatitis B virus offered for all health care providers, Changing of gloves during blood collection and tests: Is It waste of time (86%), All patients should be tested for HBV before they receive health care (85%) participants agree with that. 91% participants screened for hepatitis B(91%), 89% got vaccinated against HBV(89%), 96% had a needle prick injury and 93% used Personal protective equipments during dealing with hepatitis B Positive patient or its sample.Conclusion: At the first medical examination, newly enrolled students and other people linked to a high-risk context such as a medical institution should be examined for immunization status. This is because the number of unimmunized persons is large, particularly against hepatitis B.