Background: A prevalent skin condition that primarily affects the face but can also affect the back and chest, acne vulgaris manifests as non-inflammatory lesions, inflammatory lesions, and variable degrees of scarring. The present study compared fractional carbon dioxide laser technique and derma roller therapy in patients with post-acne scars. Materials & Methods: 64 patients with post-acne scars of both genderswere classified into 2 groups of 32 each. Group I patients was treated with derma roller therapy and the other group was treated with fractional CO2 laser every 4 weeks over a period of 24 weeks. Each patient had a baseline evaluation using the global acne scarring categorization, which was both objective and subjective. Results: Response was satisfactory seen in 7 in group I and 2 in group II, good in 9 and 10, very good in 11 and 13 and excellent in 5 and 7 in group I and II respectively. The difference was significant (P< 0.05). The mean objective score at baseline in group I was 28.2 and at follow up was 15.6 and in group II at baseline was 28.9 and at follow up was 15.1. The difference was significant (P< 0.05). Conclusion: Both the techniques were shown to be equally efficacious in therapy of post acne scars.