Background: Critically ill patients on mechanicalventilation are always at a higher risk of acquiring ventilator-associated respiratory infections. The current study was intended to determine the antibiotic-resistance pattern of bacteria recovered from the tracheal and endotracheal (ET) specimens of ventilated patients. Aims & objectives: To isolate and identify bacteria from tracheal and endotracheal aspirates and to determine the antimicrobial susceptibility of organisms isolated from tracheal and endotracheal aspirate specimens. Methodology: A descriptive study was conducted in the Department of Microbiology, Guru Gobind Singh Medical College, Faridkot for a period of 6 months. All the tracheal and endotracheal specimens collected were inoculated directly on blood and MacConkey’s agar and incubated at 37C for 24-48 hours. Results: Maximum samples 18(36%) obtained were from the age group of 0-10 years. the common isolate was Acinetobacter spp. and all isolates were resistant to ampicillin and third generation cephalosporins. The isolates of P. aeruginosa showed resistance to ampicillin and third generation cephalosporins (ceftazidime 66.6%,cefepime 33.3%) followed by amikacin(66.6%), E.coli isolates were resistant to third generation cephalosporins,,ciprofloxacin and ampicillin(100% each). Colistin was found to be most effective antimicrobial against these gram negative isolates in our study. Conclusion: Gram-negative organisms were the most common isolates from the secretions. All these isolates showed high levelsof resistance to commonly used antimicrobial agents. This poses a serious therapeutic challenge as it limits the therapeutic options available.