Background: Glaucoma is a chronic and progressive condition characterized by degeneration of the optic nerve, which can be differentiated from other types of acquired optic neuropathy by the distinctive morphology of the optic nerve head. The present study was conducted for assessing efficacy of dorzolamide 2%/timolol 0.5% fixed combination therapy in patients of primary open angle glaucoma. Materials & methods: 20 Patients having unilateral/bilateral primary open angle glaucoma diagnosed by tonometry and gonioscopy.Cases of established POAG are eligible for the study if the IOP is >21 mm Hg after discontinuation of all ocular hypotensive medication for a wash out period. 20 patients diagnosed with POAG werebe selected. Baseline IOP was recorded. The eye that was affected was considered as the study eye. IOP readings will be taken from the study eye with the Goldmannapplanation tonometer (GAT) at each visit. All the results were recorded in Microsoft excel sheet and were subjected to statistical analysis using SPSS software. Results: A total of 20 patients were evaluated. Mean age of the patients was 41.8 years. Majority proportion of patients were males. 70 percent of the patients were of urban residence. Mean IOP at baseline, first follow-up and second follow-up was 27.3 mm of Hg, 17.9 mm of Hg and 15.1 mm of Hg respectively. Conjunctival hyperemia, eye hyperemia, eye irritation and taste perversion was seen in 20 percent, 5 percent and 10 percent of the patients respectively. Conclusion: Significant improvement in IOP occurs among POAG patients undergoing dorzolamide 2%/timolol 0.5% fixed combination therapy.