Background: White spot lesion (WSL) is the earliest macroscopic evidence of enamel caries and is commonly seen during orthodontic therapy. The present study assessed the prevalence of WSL among patients who underwent fixed orthodontic treatment with passive self-ligation mechanotherapy. Materials & Methods: 88 patients in the age range of 10-30 years who received orthodontic treatment using the Damon self-ligating system of both genders were selected. The presence of WSL was investigated. Modified Gorelick’s scale was used for scoring the lesions. Results: Out of 88 patients, 32 were males and 56 were females. A non- significant difference in occurrence of WSL between different teeth of males and females (P> 0.05). The prevalence of WSL was 75% in males and 78.5% in females. Conclusion: The prevalence of WSL was 75% in males and 78.5% in females. During the first few months of treatment, doctors should assess the patients' dental hygiene habits and, if necessary, take further precautions to prevent demineralization.