Background: Furcation repair materials in the past have included amalgam, gutta-percha, calcium hydroxide, and calcium sulfate. The present study was conducted to compare sealing ability of mineral trioxide aggregate, biodentine with and without bioactive glass as furcation repair materials. Materials & Methods: 60 extracted human maxillary molars which were decoronated 3 mm above the cementoenamel junction and 3 mm below it. The samples were then divided into 4 groups of 15 each, Group I: MTA, Group II: Biodentine, Group III: BG + Biodentine and Group IV: BG + MTA and the defect was treated with respective furcation repair material. Results: The mean spectrophotometric dye absorbance values in group I was 0.43, in group II was 0.57, in group III was 0.84 and in group IV was 0.72. The difference was significant (P< 0.05). Conclusion: The sealing ability of MTA was superior to that of Biodentine, whereas the sealing ability of BG with Biodentine was superior to that of BG with MTA.