Background: Venous thrombosis can manifest as either superficial venous thrombosis or deep venous thrombosis (DVT). Deranged lipid profile is also one of the risk factors responsible for Venous Thrombosis.Hence; the present study was conducted for assessing Serum Lipid Levels and the Risk of Venous Thrombosis. Materials & methods: A total of 50 healthy controls and 50 subjects with presence of venous thrombosis were enrolled. Complete demographic and clinical details of all the patients was obtained. Blood samples were obtained in the morning and serum lipid profile was evaluated. Serum levels of cholesterol and triglycerides were assessed using a standardized enzymatic procedure. All the results were recorded in Microsoft excel sheet and were subjected to statistical analysis using SPSS software. Results: Mean age of the patients of the control group and study group was 50.9 years and 53.1 years respectively. Majority proportion of subjects of both the study groups were males. Among study group, diabetes, positive smoking habit and hypertension was seen in 30 percent, 32 percent and 26 percent of the patients respectively. Significantly lower levels of LDL-cholesterol levels were seen among subjects of the study group. Dyslipidemia was seen in higher frequency among subjects of the study group in comparison to the control group. Conclusion: Deranged lipid profile is a significant risk factor for occurrence of venous thrombosis. Hence; timely screening of lipid profile should be done.