Introduction: India has the highest burden of Acute Coronary Syndrome patients globally. The measurement of left ventricular ejection fraction (LVEF) after myocardial infarction is a class I clinical practice guideline recommendation, with prognostic and therapeutic implications. Cardiac troponin I is a sensitive and specific marker for myocardial damage, and serum ferritin, an acute-phase reactant, is considered a risk factor for MI. Aims and Objectives: To determine cardiac troponin I and serum ferritin levels in patients with first episode of STEMI, to estimate left ventricular ejection fraction in patients with first episode of STEMI and to correlate cardiac troponin I levels and serum ferritin levels in patients with first episode of STEMI with left ventricular ejection fraction. Material and Methods: This cross-sectional study was conducted in a hospital setting in the Medicine Department at GGSMCH Faridkot. 75 participants were included based on the inclusion and exclusion criteria during an 18-month timeframe. Results: The study demonstrated that in STEMI patients, lower LVEF is associated with higher levels of both troponin I and serum ferritin. A significant inverse relationship was found between LVEF and troponin I levels and a moderate negative correlation was observed between LVEF and serum ferritin. Conclusion: Assessing LVEF, cardiac troponin I and ferritin levels in STEMI patients provide valuable insights into myocardial damage, cardiac function and inflammatory status. The strong inverse correlation between cardiac troponins and LVEF underscores the importance of early and aggressive reperfusion therapies to minimize myocardial damage and preserve cardiac function.