Introduction: Iron deficiency is the most common cause of anaemia in pregnancy worldwide and is associated with increased risk of preterm births and low birth weight infants. Aim of the study: To Correlate the serum ferritin level with red cell indices and haemoglobin in pregnant women in the second and third trimester Material & Methods: The present study conducted among 100 consecutive pregnant women in the second and third trimester (12 to 40 wks) & 50 non pregnant age matched healthy controls were enlisted. 150 women attending to the OPD of NCR Institute of Medical Sciences, Meerut, U.P, India is included for this study. The blood sample was used for estimation of Hb and the red cell indices on haematology cell counter. Serum ferritin level was estimated on automated Minividas instrument using the Enzyme Linked Fluorescent Assay technique. Results: The mean age of 100 consecutive pregnant women included in our study was 21.54 years (SD = 2.73). It was found that 54 had anaemia and 46 were non-anaemic. Iron deficiency was found in 36 ladies in anaemia group (36/54) and 22 ladies in non-anaemic group (22/46) in pregnant women. The red cell indices were correlated with Serum Ferritin, it showed statistically significant results using Spearmen Coefficient (r) with Hb level, MCV, MCH and HCT. Conclusion: Thus, the international norms were found to be applicable to the pregnant Indian women and to similar populations with same stage of development as India. Also, we have concluded that we have to look for newer surrogate markers for diagnosis of sub-clinical iron deficiency in Indian women.