Introduction: Bloodstream infections (BSIs) are a major cause of morbidity and mortality in critically ill patients. This study aimed to analyze the microbiological profile and antibiotic susceptibility patterns of bacterial isolates from blood cultures in ICU patients of a tertiary care hospital in North India.Methods: A retrospective analysis of blood culture reports of ICU patients from January2022toDecember 2022 was conducted. The identification of bacterial isolates and their antibiotic susceptibility patterns were performed using standard microbiological techniques.Results: The study analyzed 66 bacterial isolates from ICU patients' blood cultures. The study found that MRSA was highly prevalent and exhibited resistance to several commonly used antibiotics, including Penicillin G, Oxacillin, Trimethoprim/Sulfamethoxazole, Erythromycin, Clindamycin and Rifampin. However, Doxycycline, linezolid, Vancomycin and Daptomycin were found to be highly effective against MRSA. The study also found that S. Aureus was resistant to Penicillin G and Erythromycin but showed sensitivity to Oxacillin, Trimethoprim/Sulfamethoxazole, Doxycycline, Clindamycin, Linezolid, Vancomycin, Daptomycin and Rifamycin. For E. coli, the study found that several antibiotics, including Amoxicillin Clavulanic Acid, Ciprofloxacin, Cefixime, Cefepime, Imipenem, and PipercillinTazobactum, exhibited resistance against all strains. However, Trimethoprim/Sulfamethoxazole, Gentamicin, Amikacin, Polymyxin B, Colistin, and Tigecycline were found to be effective and sensitive against different strains of E. coli.Conclusion: The study found a high prevalence of S. aureus, E. coli, and MRSA, with varying resistance patterns to commonly used antibiotics. The findings of this study provide valuable information for the appropriate management of BSIs in ICU patients.