Background: Acne vulgaris is a common skin condition that primarily affects the face, back, and shoulders. The present study was conducted to assess efficacy of clindamycin 1% nano-emulsion gel formulation for the treatment of Acne Vulgaris. Materials & Methods: 74 patients of Acne Vulgaris of both genders were divided into 2 groups of 37 each. Group I patients received treatment with clindamycin nano emulsion gel formulation and group II, the active control i.e. a marketed conventional clindamycin gel formulation. The total number of lesions, including inflammatory lesions (papules, pustules, nodules and cysts) and non- inflammatory lesions (open and closed comedones) were recorded. Results: With inflammatory lesions, there was 74% reduction in group I and 61% in group II. With non- inflammatory lesion, there was 65% reduction in group I and 47% reduction in group II patients. The difference was significant (P< 0.05). Outcome in group I and group II was excellent in 92% and 85%, good in 8% and 13% and fair in 0 and 2% respectively. The difference was significant (P< 0.05). Conclusion: Clindamycin 1% nano-emulsion gel formulation has demonstrated a trend towards a superior tolerability profile and is more effective than its conventional formulation.