Background:Over the past few decades, enterococci—Gram positive, facultative anaerobic cocci—have changed from being common intestinal commensals in humans and animals to serious nosocomial infections linked to considerable morbidity and mortality. The present study was conducted to assess infections caused by Enterococcal species and their antibiotic resistance pattern with respect to High Level Gentamicin Resistance (HLGR) and Vancomycin Resistant Enterococci (VRE). Materials & Methods:86 clinical isolates of Enterococci were selected. The isolates were identified, speciated using standard methods and antibiotic susceptibility was determined by Kirby Bauer disc diffusion method; and Vancomycin MIC was determined by E-test method. Results: Out of 86 samples, 21 were isolated from urine, 40 from pus, 17 from blood, 6 from synovial fluid. E. faecalis was isolated in 63, E. faecium in 12, E. raffinosus in 3, E. durans in 6 and E. avium in 2 cases. Sensitive strains and resistant strains of Penicillin was 35% and 65%, Ampicillin was 62% and 38%, Vancomycin was 94% and 6%, Gentamicin (10 µg) was 75% and 25%, high level gentamicin (120 µg) was 68% and 32%, Nitrofurantoin (for 18 urinary isolates) was 84% and 16%, Ciprofloxacin was 72% and 28%, Erythromycin was 21% and 79% and Linezolid was 100% respectively. The difference was significant (P< 0.05). Conclusion: More drug resistance to E. faecium isolates was found in the study, with a high rate of resistance to Aminoglycosides, Vancomycin, Penicillin, and Ciprofloxacin. This underscores the urgent need for more prudent use of antimicrobials and infection control.