Background: A common degenerative spinal condition that causes significant morbidity is lumbar disc prolapse. The degree of disc prolapse is largely determined by genetic predisposition. This study examines the relationship between middle-class Indians' COL9A2 gene variants and the degree of lumbar disc prolapse. Methods: Two COL9A2 variants, rs2070873 and rs144520236, were genotyped in a cohort of 100 patients who had been diagnosed with lumbar disc prolapse. Disc prolapse severity was classified as mild, moderate, or severe according to imaging and clinical standards. The association between the severity of lumbar disc prolapse and the COL9A2 variants was examined using statistical analysis. Results: One patient had severe disc prolapse out of the 100 patients with the COL9A2 variant rs2070873; the remaining 56 patients had mild disc prolapse, 43 had moderate disc prolapse, and 56 patients had neither. On the other hand, out of the 100 patients who had the COL9A2 variant rs144520236, 66 had severe disc prolapse, 23 had moderate prolapse, and 11 had mild prolapse. Based on statistical analysis, it was found that the COL9A2 variant rs144520236 significantly correlated (p-value < 0.05) with severe disc prolapse, with most carriers exhibiting severe symptoms. This implies that patients who carry this variant are significantly more likely to experience severe disc prolapse. Conclusion: The middle segment of the Indian population has a strong correlation between severe lumbar disc prolapse and the COL9A2 gene variant rs144520236. This genetic variation may be a useful marker for estimating the degree of disc degeneration, which could help with early detection and focused treatment plans for those who are more susceptible.