Background: A surgical site infection (SSI) is an infection that appears at or near the site of the surgical incision. The present study was conducted to compare single-dose antibiotic versus conventional 5 days antibiotic in clean surgery.Materials & Methods:The study was conducted in department of general surgery at ANNMCH, Gaya, Bihar during November 2021 to May 2022. 60 cases of both genders were divided into 2 groups. Group I, the antibiotic dose was given an hour prior to the surgery intravenously. In Group II, the first dose was given after the surgery for five days, wherein two days it was given intravenously and for the next three days oral tablets were given (Cefixime 200 mg bd). Incidence of SSI was recorded. Results: Group I had 14 males and 16 females and group II had 13 males and 17 females. Types of surgery was lap appendectomy in 15 and 13, lap cholecystectomy in 10 and 8, hernioplasty in 4 and 6 and breast lump (benign) in 1 and 3 in group I and II respectively. Fever was present in 6 and 8, redness was present in 7 and 9, swelling was present in 5 and 8, wound discharge was present in 5 and 6 and surgical site infection was present in 4 and 5 patients in group I and II respectively. The difference was non- significant (P>0.05).Conclusion: Single-dose antibiotic prophylaxis is a sufficient procedure for clean-contaminated surgeries.