Introduction Open fracture wounds are highly susceptible to contamination and infection, posing a significant challenge for orthopaedics. Identifying the specific bacteria present in the wound is crucial for effective antibiotic treatment and wound management. This study investigates the microbiological profile of open fracture wounds through culture analysis of wound debridement and assesses the incidence of SSIs, aiming to inform strategies for preventing post-operative infections and improving patient outcomes. Materials and Method: This study included patients with open fractures admitted to the orthopaedic department with fracture duration less than one week, and fit for anaesthesia and surgery. Patients were consecutively enrolled, and data was collected using a pre-designed proforma, with follow-ups at 1 and 3 months post-operation. The study examined various variables, including surgical wound, clinical examination findings, laboratory results, and complications. Results: The majority of subjects were male (80%) and belonged to the 31-40 age group. The study identified various organisms during debridement, with S. Aureus being the most common. The most frequently performed operation was plating (31.4%), followed by Ex Fix F/B Plating (28.57%). Post-operative signs of infection were observed in a few subjects within the first 30 days, but significantly improved after 3 months, with 97.14% showing no signs of infection. The study also tracked TLC and CRP levels pre-operatively and at follow-up, indicating a positive outcome. Conclusion: By analyzing the culture results and monitoring patients for signs of infection, the study provided valuable insights into the management of open fracture cases and the prevention of surgical site infections, ultimately informing strategies to improve patient outcomes.