Background: A long-term infection of a portion or the entire middle ear cleft, chronic suppurative otitis media (CSOM) is characterized by ear drainage and a persistent perforation. The present study was conducted to assess bacteriological causes of CSOM and to know their antibiotic sensitivity pattern. Materials & Methods: 56 cases of CSOM of both genders were selected. In accordance with CLSI recommendations, antibiotic sensitivity testing was carried out using the Kirby-Bauer disc diffusion method. Results: Out of 56 patients, males were 32 and females were 24. Organisms were Staph. Aureus in 16, Pseudomonas in 13, Klebsiella in 9, Proteus mirabilis in 6, E. coliin 4 and no organism in 8 cases. Clinical symptoms were pain in 51 and purulent discharge in 36. SESwas upper in 8, middle in 12 and lower in 36 cases. Side was unilateral in 21, bilateral in 15 and both in 20 cases. The difference was significant (P< 0.05). Antibiotic susceptibility of Staphylococcus aureus and Pseudomonas against Amikacin was seen in 75% and 80%, Amoxicillin-clavulanate in 92% and 94%, Ceftriaxone in 91%and 90%, Ciprofloxacin in 84% and 77%, Cotrimoxazole in 55% and 67%, in Cefoperazone + sulbactam in 87% and 90%, Levofloxacin in 46% and 83%, Nitrofurantoinin 23%and 49%, Metronidazole in 51% and 78% and Piperacillin +tazobactam in 75%and 46% respectively. The difference was significant (P< 0.05). Conclusion: When it comes to ear infections, there may be differences in the pathogens and how susceptible they are. Proteus, E. coli, Klebsiella, S. aureus, and Pseudomonas were the most often isolated species in ear infections in our investigation.