Background and objective: Dermatophytes, categorized into Trichophyton, Epidermophyton, and Microsporum, are hyaline molds with protease enzymes that enable skin colonization, invasion, and infection of the outer layers, hair shaft, and nails. The objective of this study is to find out the association of family history and gender with dermatophyte infection. Material and method: A cross-sectional investigation involved 404 clinically suspected dermatophytosis cases at Maharaja Yashwantrao Hospital, Madhya Pradesh. Samples were sent to the Department of Microbiology between December 2022 and January 2023, with permission from both scientific and ethical committees.Result: In the present study, a total of 404, clinically suspected cases of dermatophytosis were included. Out of which, 204 (50.5%) were females and 200 (49.5%) were males. Out of 404 cases, 205 (50.7%) dermatophytes were isolated, in which 112 (54.6%) were Trichophyton rubrum, 92 (44.8%) were Trichophyton mentagrophytes and 1 (0.48%) was Microsporumgypseum.Conclusion: The study found that dermatophyte infection is more common in males due to outdoor activities and labour work, but no significant association was found between family history and infection.