Aim: To assess the lipid profile of individuals following laparoscopic cholecystectomy. Materials and Methods: 100 gallstone patients were included in this study.The demographic profile and complete medical history of all patients were documented. Preoperative physical examinations were conducted. Blood samples were collected under septic conditions two hours before surgery to assess the preoperative lipid profile. Laparoscopic cholecystectomy was performed by skilled and experienced surgeons. Postoperative blood samples were collected one week after surgery and sent to the laboratory for lipid profile analysis.The serum lipid parameters analyzed included total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and triglycerides (TGs). Results: The analysis of serum lipid profiles indicated a significant reduction in both total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) levels postoperatively. The mean preoperative TC level was 190.5 mg/dL, which decreased to 175.3 mg/dL postoperatively (p=0.01). Similarly, the mean HDL-C level decreased from 48.7 mg/dL preoperatively to 45.6 mg/dL postoperatively (p=0.04). However, the change in triglyceride (TG) levels was not statistically significant, with preoperative levels at 155.8 mg/dL and postoperative levels at 160.4 mg/dL (p=0.12).When analyzed by gender, both male and female patients exhibited significant reductions in TC levels postoperatively. For males, the TC level decreased from 192.3 mg/dL to 177.1 mg/dL (p=0.02), and for females, it decreased from 188.7 mg/dL to 173.5 mg/dL (p=0.03). The reduction in HDL-C levels was not statistically significant for males (from 47.2 mg/dL to 44.9 mg/dL, p=0.06) but was marginally significant for females (from 50.2 mg/dL to 46.3 mg/dL, p=0.05). Triglyceride levels increased slightly in both genders but did not reach statistical significance. Conclusion: These results suggest that laparoscopic cholecystectomy significantly impacts serum lipid profiles, particularly in reducing total cholesterol and HDL cholesterol levels. The lack of significant change in triglyceride levels indicates that the procedure's effect on this parameter may be more variable and warrants further investigation.