Aim: To determine the disease activity score in rheumatoid arthritis in relation to vitamin D levels. Materials and Methods: The present study consisted of 70 patients of rheumatoid arthritis of both genders. All gave their written consent to participate in the study. Data such as name, age, gender etc. was recorded. Parameters such as onset of symptoms, disease progression, pattern of joint involvement, pain and swelling in joints etc. was recorded. Investigations i.e. ESR, Rheumatoid factor (Quantitative) and C - reactive protein (Quantitative) were carried out. Measurement of vitamin D was done with ELISA in an autoanalyzer. Disease activity score 28 (DAS28) and Visual analog scale scoring system tools were used. For calculating the DAS28, swollen tender joint examination was performed and the value of each affected was noted. Disease severity was evaluated according to the significance of DAS28 score are as follows- Remission: DAS28 ≤2.6, Low disease activity: 2.6 5.1. Data thus obtained were subjected to statistical analysis. P value < 0.05 was considered significant. Results: Mean vitamin D level in patients with low disease activity was 10.85 ng/ml, in moderate disease activity was 11.63 ng/ml and in high disease activity group was10.78 ng/ml. The mean DAS- 28 score in low, moderate and high disease activity group was 2.31, 4.89 and 5.16 respectively. The difference was significant (p<0.05). Conclusion: we found that a significant association has been found between the comparison of the level of vitamin D and the DAS-28 score.