Introduction: Dengue is a mosquito transmitted arboviral infection and leading cause of arthropod borne viral disease in the world. Leptospirosis is a zoonotic disease distributed worldwide affecting almost 160 mammalian species. The similarity in their clinical presentation, may result in the possibility of coinfection going undetected. Aim: To determine the prevalence of dengue and leptospirosis coinfection at a tertiary care hospital. Materials and Methods: Serum samples were received from the clinically suspected cases of dengue fever for a period of 4 months. These samples were tested Dengue Immunoglobulin M (Ig M) antibodies using National Institute of Virology (NIV) IgM ELISA Kit and Dengue positive samples were further tested by Leptospira Ig M ELISA kit (RecombiLISA). Results were interpreted according to the manufacturer instructions. Results: Among 749 samples, 79 (10.54%) samples were positive for dengue IgM antibody. Among these dengue positive cases 3 (3.8%) were positive for leptospirosis and dengue co infection. Among which 2 (66.6%) were males and 1 (33.3%) was female. The common age group of people affected with coinfection belongs to 20-30 years. Conclusion: This study emphasizes the possibility of coinfection of leptospirosis and dengue in fever cases. Therefore, high index of clinical suspicion is required so as to constitute appropriate therapy to reduce the mortality and morbidity rate.