Background: The relationship between serum paraoxonase enzyme activity and dyslipidemia in obese individuals has received a lot of attention lately, demonstrating the interdependence of lipid profiles and enzymatic performance in the obesity milieu. The present study was conducted to assess serum paroxanase enzyme activity in obese subjects. Materials & Methods: 150 obese subjects of both genders were enrolled. Serum paraoxonase activity was measured using a spectrophotometric enzymatic method, and lipid profiles were determined using automated enzymatic assays. Results: Out of 150 patients, 85 were males and 65 were females. The mean BMI was 31.6 kg/m² in males and 33.4 kg/m² in females. The mean age was 46.3 years in males and 47.1 years in females. The mean serum paraoxonase activity was 63.9 U/L in males and 61.4 U/L in females. The difference was significant (P< 0.05). There were 35% male and 24% female smokers and 65% male and 76% female non- smokers.There were 29% male and 18% female drinkers and 71% male and 82% female non- drinkers. Physical activity was sedentary in 56% male and 68% females and active 44% male and 32% females. The difference was significant (P< 0.05). Conclusion: Serum paroxanase enzyme activity in obese subjects found to be lowered.